A predictive 3D multi-scale model of bile flux in the liver lobe
IT / Software / Bioinformatics : Bioinformatics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Cell related
Our scientists have developed the first predictive 3D multi-scale model that simulates bile flow from the sub-cellular level to the tissue level. The model integrates structural and functional properties of the mouse liver lobule determined from high-resolution confocal, intravital and serial block-face scanning electron microscopy, as well as image analysis techniques.
An important new finding of this model is that osmotic pressure alone is insufficient to explain bile flow, and that bile canaliculi contractility is an additional key component. Moreover, using Acetaminophen as an example, our scientists demonstrated the precision of the model, that enabled detection of phenotypes that had previously only been detected for toxic doses of Acetaminophen in liver tissue.
It is also expected to be translatable to human liver; functional tests of bile acid clearance using fluorescent bile tracers, as previously tested in patients, may offer a strategy to calibrate this model to human biliary fluid dynamics.
The imaging and image analysis approaches used are based on a working pipeline that reaches medium throughput, making this model suitable for clinical and pharmacological studies.
Strategy of a Multi-Scale Model of Biliary Fluid Dynamics
We are now looking for either a licensing partner, or a collaboration partner to further develop this project.
The PCT application was filed on February 20, 2018.
- Ref.-Nr.: 1305-5341-MSG (347.6 KiB)
Dr. Ingrid Kapser-Fischer
Phone: +49 89 / 29 09 19-19