Strep-tag technology: simple protein purification
The method works by attaching a short peptide sequence, the so-called strep-tag, to the protein during production. The tag has a high affinity to streptavidin, a well-established lab reagent. If the protein solution is then poured onto a purification column in which Strep-Tactin, a streptavidin analogue is bound to the column material, the desired protein is selectively separated.
The technology was out-licensed by Max Planck Innovation to IBA GmbH in Göttingen. In the meantime, Strep-tag is the world's leading tool for the purification of proteins.