Imaging and Microscopy

Extended search



Cryo-Light Microscope and Immersion Medium for Cryo-Light Microscopy

Ref.-No.: 0707-5496-BC

Imaging and Microscopy

Cryogenic fluorescent light microscopy of flash-frozen cells presents significant advantages and provides an important complement to electron cryo-microscopy. In particular, bleaching decreases drastically at low temperature while the fluorescence yield of many fluorophores increases and the spectral bands narrow. The application of modern super-resolution methods such as STED, PALM, STORM, or SIM at cryogenic temperature holds the prospect of imaging fluorescent proteins with high precision in ...

Digital scanner

Ref.-No.: 0105-5505-BC

Imaging and Microscopy

Light microscopy enables fascinating insights into the microcosm of life. It is one of the key techniques in life science and provides access to a better understanding of biology across many orders of magnitude in time and space. The use of visible light allows for minimally invasive imaging - even of living cells - and direct observation with the human eye. However, the use of visible light limits the resolution of the image obtained to about half the wavelength of the used light. The limiting ...


Ref.-No.: 1305-5612-WT

Imaging and Microscopy
Sensors, Devices and Components

Dynamic Parallel Imaging: New Method for Accelerated MR Imaging

Ref.-No.: 0107-5654-BC

Imaging and Microscopy

The invention relates to a new method to reduce acquisition time in MR imaging. It is based on the variation of the sensitivity profile of the RF coils and allows an easy access to an additional source of spatial information to further improve imaging.
MR imaging is among the most important tools for modern medical diagnostics with a unique range of applications. The imaging duration however limits the applicability for situations with movements within the volume of interest. Therefore, many ...

Electron Microscopy: Ready to Use AI for Focus and Astigmatism Adjustment

Ref.-No.: 0202-6337-BC

Imaging and Microscopy

The invention relates to a new method for imaging setting optimization for electron microscopy
based on deep learning. The AI is a robust and simple to implement optimization possibility for
direct operation with various types of electron microscopes.

For a wide spectrum of applications automated precision electron microscopy is a powerful
method. Existing auto focusing routines are often based on physical models of the microscope
and are hence lacking general applicability ...

Faster Processing: Neural Network for MR Imaging Parameter Prediction

Ref.-No.: 0107-6054-BC

Imaging and Microscopy

The invention relates to a new method for processing magnetic resonance (MR) data based on a neural network (NN). After training, the NN quickly delivers high quality CEST images with low computational effort. Additionally, it provides uncertainty data aiding to judge the images appropriately.

Typically, sophisticated MR contrasts are calculated based on complex mathematical models, which is computationally expensive, and it requires long processing durations. Besides that, the results ...

Flash 2 – Real-Time Magnetic Resonance Imaging (MRI)

Ref.-Nr.: 0707-4198-BC

Imaging and Microscopy
Sensors, Devices and Components

Magnetic resonance imaging (MRI) is nowadays a leading mo¬dality for diagnostic imaging with about 100 million examinations per year worldwide. However, when invented by Paul Christian Lauterbur in 1973, MRI was too slow to allow for routine medical applications. A breakthrough was achieved in 1985 by the FLASH (Fast Low Angle Shot) technique developed by Jens Frahm and his team at the Max-Planck-Institute for Biophysical Chemistry in Göttingen, Germany. With FLASH the measuring time for a cross-sectional ...


Ref.-No.: 1629-4734-WT

Imaging and Microscopy
Sensors, Devices and Components

Localized Oscillating Magnetic Fields: New Method for Accelerated MR Imaging

Ref.-No.: 0107-5557-BC

Imaging and Microscopy

The invention relates to a new method to reduce acquisition time in MR imaging. It is based on the use of localized temporal modulations of the main magnetic fields which provides further spatial information from the resonance signal. The method can be applied to parallel imaging.
MR imaging is among the most important tools for modern medical diagnostics with a unique range of applications. The imaging duration however limits the applicability for situations with movements within the volume ...

Microfluidic cryo-fixation of biomass, e.g., cells, flagella/cilia, or proteins for (in particular) correlative light-electron microscopy systems

Ref.-No.: 0707-4032-BC

Imaging and Microscopy

Cryo-Electron Microscopy (Cryo-EM) today is revolutionizing biological research by revealing the structure of cells and proteins with atomic resolution and steadily improving contrast. However, the quality of the results depends critically on the sample preparation. High Pressure Freezing (HPF) and plunge freezing, both pioneered in the 1960s, still represent the state-of-the-art in sample preparation for cryo-EM. Both methods have poor timing precision, disrupt the natural environment of the ...

Microscopy: Differentiable model-based adaptive optics with transmitted and reflected light

Ref.-No.: 2022-6511-FG

Imaging and Microscopy

The invention relates to a method and illumination device for optimizing parameters of a physical light propagation model, in particular a light propagation model in a confocal laser microscope using a raster scanning method. The method can determine aberration corrections that go beyond a predetermined model of aberrations, such as combina-tions of Zernike modes.

MINSTED fluorescence localization and nanoscopy

Ref.-No.: 0707-6171-BC

Imaging and Microscopy

The MINSTED method is an innovative technique for determining the position of a singularized fluorophore molecule within an object. Traditional methods in molecular imaging faced challenges in achieving high resolution due to limitations in fluorescence microscopy. Existing solutions like STED (Stimulated Emission Depletion) microscopy improved the spatial resolution but rely on high illumination intensity and/or sample exposure, which can lead to thermal issues and photochemical reactions. MINSTED ...

Multichannel coil for UHF MRI

Ref.-No.: 0107-4677-BC

Imaging and Microscopy
Sensors, Devices and Components

Exploiting the benefits offered by magnetic resonance imaging (MRI) at ultra-high fields (≥ 7 Tesla) requires optimized radiofrequency (RF) coils. MRI at UHF operates in a regime where the RF wavelength is comparable to the dimensions of the sample size, resulting in an inhomogeneous distribution of the transmit field (B1+) and in an impaired image quality. An array of independent transmit coils arranged in multiple rows provides the degrees of freedom to influence the ...

Nanographene-based dyes as high performance probes for super-resolution microscopy

Ref.-Nos.: 0903-5658-LC; 0903-5696-LC

Imaging and Microscopy
New Materials

A group of scientists at the MPI for Polymer Research in Mainz developed nano-graphene structures that can be used as a new class of fluorophores for super-resolution microscopy (SMLM and STED).

They perform like hybrids of organic dyes and QDs: they have excellent environment-independent blinking properties, emit high photon numbers, display high photo-stability, possess low toxicity, have a molecular size below 2 nm, and have narrow excitation and emission spectra.

These nanographene ...

Noise Free Imaging: Incoherent Light Source for Microscopy

Ref.-No.: 1629-5817-WT

Imaging and Microscopy

The invention relates to a new light source exhibiting a high level of spatial incoherence. This enables microscopic imaging with reduced artefacts and low intensity noise. It comprises a pulsed laser whose beam properties are improved is by a multimode optical fiber.
Full field microscopic imaging is an essential technique in medicine and beyond, but the occurrence of noise strongly limits its applicability. Coherent light contributes considerably to the formation of artefacts. Hence the ...

Parallel-imaging time-of-flight momentum microscope

Ref.-No.: 1401-4572-WT

Imaging and Microscopy
Sensors, Devices and Components : Devices

The state of an electron is completely defined by its momentum and spin. In processes like electron diffraction or photoemission electron ensembles are generated which have a characteristic 3D distribution of momentum and spin. To examine such events the three components of the momentum vector have to be measured. Up to now, the momentum distribution of an ensemble of charged particles was typically measured using energy-dispersive spectrometers based on sequential approaches (sequentially changing ...

PONy Dyes – Fluorescent Dyes with Phosphorus Substituents

Ref.-No.: 0707-5297-BC

Imaging and Microscopy
New Materials

Fluorescent dyes are widely used as indispensable markers in biology, optical microscopy, and analytical chemistry. In particular, the sensitive and stable imaging of cellular components depends on the favourable combination of chemical, biological and physical factors. The availability and proper choice of fluorescent dyes is a key factor to success in the entire labelling and imaging procedure. Due to their superior brightness and photostability, synthetic dyes represent an attractive alternative ...

Precision Microscopy: Detector for Changes in Direction of a Light Beam

Ref.-No.: 0105-5623-BC

Imaging and Microscopy

The invention relates to a new method for light beam angle detection that is based on interferometry. High precision and sensitivity for slight changes of the beam direction can be achieved with this method.
For high precision light microscopy, the light beam must be aligned accurately, and the beam direction stability must be ensured in order to maintain a sufficient imaging resolution over longer periods of time. In standard microscopy environments even small temperature changes can result ...

Reflection Microscopy: Ready to Use Neural Networks for Wavefront Correction in Adaptive Optics

Ref.-No.: 2022-6510-FG

Imaging and Microscopy

The invention relates to a method for wavefront correction in adaptive optics compatible with laser scanning microscopy based on reflected light and deep neural networks. For network training sample aberrations are generated in the excitation and detection path by means of spatial light modulators, and the corresponding reflected focus images are recorded by CMOS cameras. After training, the network can disentangle and independently correct excitation and detection aberrations. The predicted aberration ...

Reliable and Independent: New Automated Glaucoma Detection Method

Ref.-No.: 0705-5468-BC

Imaging and Microscopy

The invention relates to a newly developed image processing method for optical coherence tomography (OCT) to be used in particular on the (human) eye’s anterior chamber. Said OCT images are used to diagnose glaucoma, but the required assessing methods are not yet available as automated and fully unsupervised.

Glaucoma is the main reason for irreversible blindness. Early-stage diagnosis is typically based on images of the anterior chamber. Various automated techniques for image analysis ...

Simultaneous multi-color fluorescence microscopy with improved spatio-temporal resolution and image contrast

Ref.-No.: 0707-5644-BC

Imaging and Microscopy

Fluorescence microscopy is one of the key techniques in the life and materials sciences, with applications ranging from routine diagnostics to state-of-the-art research investigations. Key to this success has been its intrinsic advantages combined with continuous improvement in its fundamental features such as resolution, speed, and sensitivity.

Two key challenges are fast single-fluorophore imaging of multi-color samples. This need arises in a broad range of settings such as very high-resolution ...

Ultrarapid cryo-fixation during live observation on a fluorescence microscope

Ref.-No.: 0803-6035-IKF

Imaging and Microscopy

A ultrarapid cryo-arrest directly on a multimodal fluorescence microscope that preserves molecular activity patterns during observation of their dynamics in living cells at any timepoint.