Nucleic Acid-, Protein and Cell-related Technologies

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A genetically conserved and transferable defense mechanism protects plants from herbivores

Ref.-No.: 1602-6322-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Green Biotech

Our scientists have identified a conserved defense mechanism covering a new metabolite in tobacco that protects against leafhoppers. They have discovered how to reconstitute it in vitro and engineer the compound in crop plants, demonstrating that it confers resistance to herbivores.

A novel inhibitor targeting matrix accumulation in cancer

Ref.-No.: 0105-5855_1-IKF

Medicine : Therapeutics
Medicine : Animal Models
Nucleic Acid-, Protein and Cell-related Technologies

Cancer remains a major health burden and is the second leading cause of death globally (WHO). The microenvironment of cancer cells plays an important role in disease progression. In particular, the composition of the extracellular matrix regulates angiogenesis, cell proliferation, differentiation, cell survival and apoptosis. Dysregulation and accumulation of the extracellular matrix promotes cellular transformation and metastasis leading to cancer progression. A better understanding of the role ...

Application of novel channelrhodopsins: molecular engineering, site-specific opsin targeting, discovery of new variants and the advancement in optogenetic devise development

Ref.-No.: 0601-4259-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...

Broad-spectrum vector-based antiviral and potential live vaccine

Ref.-No.: 1402-5459-LC

Medicine
Nucleic Acid-, Protein and Cell-related Technologies

Our scientists developed a vector-based high tolerable and efficient antiviral (plasmid-derived defective interfering particles) to support IAV pandemic preparedness. The DIP also shows a broad-spectrum antiviral activity against other respiratory viruses, e.g. RSV and SARS-CoV-2 and is Intended for intranasal administration (droplet spray) for human use. A state-of-the-art, high-yield production process in a GMP-ready controlled and sterile environment in animal cell culture.

f-Chrimson and vf-Chrimson, two ultrafast variants of the red-light activated channelrhodopsin Chrimson

Ref.-No.: 0601-5208-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues, and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...

FFA2 (GPR43) and FFA3 (GPR41) as novel targets for antidiabetic therapies

Ref.-No.: 0603-4987-IKF

Medicine : Therapeutics
Medicine : Animal Models
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

A double KO, a conditional KO as well as single KO mouse models for FFA2 and FFA3 (free fatty acid receptors 2 and 3), novel targets for diabetes therapies.

Generation of induced pluripotent stem cells with one or two reprogramming factors

Ref.-No.: 1012-3916-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The cellular identity of mature cells that are confined in a specialized genetic expression profile can be overcome by introducing just a few genes that encode for so-called reprogramming factors [1]. By means of this intervention, also known as reprogramming, the cells then become pluripotent and represent induced pluripotent stem cells (iPS cells) [1]. Nowadays, numerous new cell culture models have been established based on reprogramming protocols that yield iPS cells. Such cell culture models ...

Generation of RNA therapeutics by conjugating NAD-capped RNAs to proteins

Ref.-No.: 0706-6285-IKF

Nucleic Acid-, Protein and Cell-related Technologies

Conjugation of a NAD-capped nucleic acid to a protein catalysed by an ART to generate novel RNA therapeutics.

Genome-Editing-Technologies - Cas9 double mutant with improved specificity compared to wild-type

Ref.-No.: 0306-5787-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

The RNA-programmable DNA-endonuclease Cas9 is widely used for genome engineering, where a high degree of specificity is required. Though this is a mechanism well controlled by canonical base-pairing, there is evidence that Cas9 does accept mismatches under certain circumstances.
This might be seen as a threat to precision needed in genome engineering at least in environments where such high precision is ultimately needed and acceptance of mismatches needs to be avoided, e.g.: cellular gene ...

Genome-Editing-Technologies - Single compound or compound mix dramatically improve Homology-Directed-Repair (HDR)

Ref.-No.: 1306-5734-LI & 1306-5288-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

Precise genome editing demands for homology-directed repair (HDR) by aiming to introduce a new double stranded DNA substituting for a homologous sequence but partially defect one. This substrate dependent reaction does compete with direct non-homologous end joining (NHEJ) of the double strand breaks. NHEJ is error-prone and is therefor mainly used for gene inactivation.
The rate of NHEJ is directly limiting the rate of HDR. For this reason HDR in vitro does occur on a very low level only and ...

Glue your proteins of interest together: Fast and ultra-specific enzymatic conjugation of a protein with another, synthetic compounds or even whole cells

Ref.-No.: 0109-5804-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

Connectase is an enzyme that fuses target proteins or synthetic compounds via a small recognition tag, using a different reaction mechanism compared to known protein ligases. It prevents side reactions entirely and has absolute specificity, even in highly in complex solutions with minimum concentrations of target molecules.

GOLD gRNA - allows robust genome editing regardless of spacer sequence composition by super stable hairpin technology

Ref.-No.: 1306-6101-LI

Processes and Methods (incl. Screening)
Medicine
Research Tools
Nucleic Acid-, Protein and Cell-related Technologies

A Cas nuclease such as Cas9 can introduce a DNA double strand break in a sequence target that is complementary to a 20-nt spacer sequence of its bound guide RNA (gRNA) when a protospacer adjacent motif (PAM) is present. The gRNA can be provided as duplex of spacer containing CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA). Or as a single gRNA (sgRNA) where crRNA and tracrRNA are fused by an artificial loop.
Hence a gRNA consists of the target-specific spacer and a constant part typically ...

Homogenous, TR-FRET-based method for measurement of RNA- and DNA-polymerase activity

Ref.-No.: 1013-4990-IKF

Analytics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

An innovative high throughput screening method for measurement of RNA- and DNA-polymerase activity at room temperature, based on time-resolved FRET.

LITESEC - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Ref.-No.: 0706-5707-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Drug delivery
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

The LITESEC-T3SS (Light-induced translocation of effectors through sequestration of endogenous components of the T3SS) system represents a novel, fast, specific, and reversible method to control protein secretion into eukaryotic cells, enabling various medicinal and biotechnological applications, such as targeted drug delivery for tumor therapy.

Method for cell-free protein synthesis assays or other fluorescent assays in the context of cell-free protein synthesis

Ref.-No.: 0204-5479-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

An improved method to perform cell-free protein synthesis (CFPS) assays or other fluorescent assays in the context of CFPS with very low reaction mix volumes. Various additional advantages allow to significantly reduce costs and time.

Molecularly defined non-infectious synthetic virus-like particles to mimic biophysical features of natural pathogens (Kopie)

Ref.-No.: 0105-6291-IKF

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

A synthetic virus-like particle, comprising a lipid bilayer, that can be adjusted precisely to mimic biophysical features of various natural pathogens. The lipid bilayer can also be equipped with relevant molecules such as the SARS-CoV-2 spike protein ectodomain.

Multi-well Exosome Libraries for High-throughput In Vitro Cell Activity Assays

Ref.-No.: 0707-6429-MG

Nucleic Acid-, Protein and Cell-related Technologies : Cell related

Extracellular vesicles (EVs) such as exosomes and microvesicles participate in the intercellular communication by transferring bioactive molecules between various cells and tissues. Such EVs cross-talk was shown to play important roles in cell–cell communication, cell maintenance, disease progression and therapy. By containing disease specific markers, EVs can also act as diagnostic biomarkers. In addition, synthetic EVs are used as carriers to deliver drugs or mRNA-based vaccines to specific ...

New cell type promoting osteogenesis in bone

Ref.-No.: 1012-4727-IKF

Medicine : Diagnostics
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

A promising target to develop treatment options for osteoporosis and other disease conditions associated with bone loss

New KDAC assay and selection system

Ref.-No.: 0803-5480-IKF

Analytics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Diagnostics
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

We present an extremely sensitive, reliable and fast novel Lysine deacetylase (KDAC) assay, which can be performed in a continuous format. This novel KDAC assay is based on Firefly luciferase harboring an acetylation on an essential active site lysine, incorporated by genetic code expansion.

We furthermore offer a bacterial selection system that can help engineer KDACs. The selection system uses a reporter enzyme inactivated by lysine modifications at an essential active site residue, thereby ...

Novel Biomarkers for Ageing and Metabolic Health

Ref.-Nr.: 1013-5354-IKF

Analytics
Medicine : Therapeutics
Medicine : Diagnostics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

Biomarkers of ageing have long been sought after to help assess the biological age and general health status of individuals. Considerable efforts have been invested to identify such biomarkers, including physiologic readouts, metabolic parameters, glycomic profiles and others. Nevertheless, markers with strong predictive power have remained elusive.

Work in model organisms has identified several conserved signaling pathways that extend life span, such as reduced insulin/IGF signaling and ...

Novel compounds for plant disease control

Ref.-No.: 0707-5356-MG

Nucleic Acid-, Protein and Cell-related Technologies : Protein related
Green Biotech

Plant pathogenic fungi, oomycetes and bacteria have highly diverse lifestyles, infection strategies and morphologies. Therefore, pesticides frequently target basic cellular processes, as these are well characterized and often very similar among plant pathogens. Many pesticides target enzymes that play a role in e.g. nucleic acid synthesis, respiration, cell division and other essential cellular processes. However, the molecular ...

Novel Imine Reductases (IREDs) for the enantioselective preparation of primary amines

Ref.-No.: 0706-5617-IKF

Nucleic Acid-, Protein and Cell-related Technologies : Protein related

Chiral amines are crucial building blocks for the synthesis of bioactive compounds in the pharmaceutical, and agrochemical industry. Chiral amines are also important for chemical synthesis as chiral auxiliaries or resolving agents for diastereometric salt crystallizations. In recent years, biocatalysts, particularly imine reductases (IREDs) and reductive aminases capable of reducing imines to chiral amines were discovered and developed for industrial application. However, the portfolio of current ...

Predicting concentrations, reactions rates and rate constants in biochemical networks

Ref.-No.: 0402-5469-MG

Analytics
IT / Software / Bioinformatics : Bioinformatics
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

A computer-implemented method that allows calculating concentration ranges of components within complex networks of biochemical reactions.

Protein Epitope Signature Tags (PrESTs) for SILAC-based absolute protein quantification

Ref.-No.: 0204-4338-MG

Nucleic Acid-, Protein and Cell-related Technologies : Protein related

Direct quantification of the absolute amount of multiple proteins in one a SILAC experiment using Protein Epitope Signature Tags (PrESTs) which consist of a short and unique region of the protein of interest as well as purification and solubility tags and are expressed recombinantly in E. coli

Stable, site-specific conjugation of His-tagged proteins via cobalt (III) carbonate reagents

Ref.-No.: 0105-5774-IKF

Analytics
Processes and Methods (incl. Screening) : Chemical
Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

This invention relates to means and methods for conjugating/attaching target molecules such as proteins to a label and/or carrier.

SUMOvera: A Truly Orthogonal Cleavage Module

Ref.-Nr.: 0402-5439-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

SUMOvera enables versatile purification strategies for broad applications in eukaryotic hosts.

Tet-inducible “gePSI” protein reversibly regulates eukaryotic ribosomal protein synthesis in a cell-type specific manner allowing to impose and release protein synthesis inhibition in a select subset of cells in mixtures

Ref.-No.: 0602-5504-LI

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Nucleic acid related

Proteins are the main functional units within cells. Their regulated synthesis and degradation are crucial for controlling biological processes. In all cells, protein synthesis is used to respond to extra- and/or intracellular cues to remodel cellular function. Previous studies probing the role of protein synthesis have used chemical inhibitors, often common antibiotics, which are effective but lack functional and cell-type specificity when applied to a mixture of cells.

Unique multifunctional oligonucleotide enabling ultrafast RNA-protein interactions in cells and other uses thereof

Ref.-No.: 0214-5029-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Nucleic acid related
Nucleic Acid-, Protein and Cell-related Technologies : Protein related

Unique multifunctional oligonucleotide for the ultrafast transcriptome-wide identification of RNA-binding protein (RBP) targets.

Xenorhodopsin - a new inward-directed proton pump for an alternative optogentic approach

Ref.-No.: 0601-5295-MG

Processes and Methods (incl. Screening) : Life Sciences-HTS/HCS
Medicine : Therapeutics
Research Tools : Other
Nucleic Acid-, Protein and Cell-related Technologies : Cell related

The development of the cation channel channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii by Prof. Ernst Bamberg and his colleagues, and its application to light-induced modulation of neurons paved the way for the field of optogenetics.
Over time the light-gated cation channel channelrhodopsin-2 (ChR2) has become an indispensable tool in neuroscience. Based on the pioneering work on ChR2 new variants have emerged, differing in their spectrum of light absorption, their kinetic ...