High-precision base editors for site-specific single nucleotide conversion
Processes and Methods (incl. Screening)
Researchersfrom the Max-Planck-Institute of Molecular Plant Physiologyin Golm invented high-precisionC-to-T baseeditors with narrow activity windows that can selectively edit a single nucleobase at a specific position with high accuracy and high efficiency(1). The scientistshypothesized that structural flexibility between the Cas9 module and the deaminase module caused the observed inaccuracies of previously designed base editors,and decided to engineertheconnectionbetween the modules. The use ofrigidifying linkersand the truncation of non-essential andflexible protein parts resulted in new fusion proteins that showa strong increase in editing precision while maintaining full deaminase activity. Thenarrow-window base editors combine superior editing precision withhigh editing efficiency and product purity,and will bewidely applicablein many areas of basic and applied research
We are seeking licensing partnersfor the further development and exploitation of this technology.
(1) Tan, J. et al., Nat Commun 2019 Jan 25; 10(1):439. doi: 10.1038/s41467-018-08034-8
A PCT application was filed in March 2019.
- Ref.-Nr.: 0402-5782-MG-ZE (123.3 KiB)
Dr. Mareike Göritz
Phone: +49 89 / 29 09 19-32