Novel method for analysis of complex carbohydrate structures by ion-mobility mass spectrometry (IM-MS)
Scientists of the Max Planck Society and of the Freie Universität Berlin developed a novel, highly sensitive and fast method for unambiguous determination of the composition, connectivity and configuration of glycans by ion mobility-mass spectrometry (IM-MS) in negative ionization mode.
By combining the rotationally averaged collision cross-section (CCS) information of an ion in a specific drift gas, a molecular property that under controlled conditions is independent of instrument parameters and that is correlated to the shape of an ion with m/z data, the structural identification of glycans and glycoconjugates can be achieved.
Sample and time requirements of IM–MS are similar to those of conventional mass spectrometry, so the additional information is obtained at no extra cost. Additionally, with this novel method samples can be analysed with concentrations as low as 0.1 % of the target carbohydrate, making it a highly sensitive approach that requires very low sample quantities.
Combined with a CCS-database this new IM–MS-based method is a powerful tool that drastically improves the accessibility to structural information of complex carbohydrates. Connectivity and configurational isomers can be separated efficiently with baseline resolution, and the relative content of isomeric impurities can be determined quickly and easily, which paves the way for routine applications like quality control or diagnostic glycan biomarkers.
- Pagel, P.H. Seeberger, J. Hofmann, H.S. Hahm: "Identification of carbohydrate anomers using ion mobility–mass spectrometry"; Nature, Vol 526, pages 241+ , 8 October 2015
- Pagel, J. Hofmann: "Glycan Analysis by Ion Mobility–Mass Spectrometry"; Angew. Chem. Int. Ed. 2017, 56, 8342 – 8349
PCT priority application filed: WO2017036545 A1,
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Dr. Mareike Göritz
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